Curcumin Protects Neuron against Cerebral Ischemia-Induced Inflammation through Improving PPAR-Gamma Function

Figure 7

Curcumin-induced PPARγ inhibited NF-κB pathway in cerebral ischemia of rats. Rats were injected (i.p.) with curcumin and GW9662 for continuous 3 days. One hour after the last injections, the rats were subjected to MCAO for 2 h and reperfused for 24 h. (a) Western blot assay of IκB-α. (b) EMSA assay of NF-κB-DNA-binding activity. (c) Western blot assay of NF-κB p65 subunit. (d) Co-IP assay of the interaction of PPARγ and NF-κB p65. Data were expressed as mean ± SD. Western blot images were representative of four independent experiments demonstrating similar results. **𝑃<0.01 versus sham-operated rats, ^##𝑃<0.01 versus MCAO rats, ^Δ𝑃<0.05, and ^ΔΔ𝑃<0.01 versus curcumin-treated rats.

(a)

(b)

(c)

(d)