Aqueous Extract of Paeonia lactiflora and Paeoniflorin as Aggregation Reducers Targeting Chaperones in Cell Models of Spinocerebellar Ataxia 3
Enhancement of chaperone expression by paeoniflorin in 293 cells. (a) Triple fluorescent reporter plasmid with HSF1, HSPA8, and HSPA1A promoter fragments upstream of mCherry, ZsYellow1, and AmCyan1 fluorescent reporters, respectively. (b) Microscopic images of the triple fluorescent reporter cells. (c) Effect of GGA and paeoniflorin (100 nM~100 µM) on HSF1, HSPA8, and HSPA1A reporters. To normalize, the fluorescence level in untreated cells is set as 100%. Three independent experiments were performed with considered significant. (d) Representative western blot image of paeoniflorin-(100 nM) treated 293 cells for two days using HSF1, HSPA8, HSPA1A, and H3F3B antibodies. Levels of HSF1, HSPA8, HSPA1A, were normalized with a loading control (H3F3B). Data are expressed as the mean ± SEM values from three independent experiments.