Primo Vascular System in the Lymph Vessel from the Inguinal to the Axillary Nodes
Figure 4
Immunofluorescence with EMP-3 of primo vessel in the lymph vessel. (a) DAPI signal image of the cross-sectioned primo vessel. Notice that only few nuclei were distributed in the region of the genuine primo vessel (arrow). This was in agreement with the structure of primo vessel with bundles of fibers outside the endothelial cells. (b) EMP-3 signal image of the cross-sectioned primo vessel. EMP-3 was positively reacted with the surrounding cells of the genuine primo vessel. These cells were epithelial cells at the boundary of the primo vessel. The very strong signals at the larger boundary of the aggregated lymphocytes were not understood yet. (c) Merged image of (a) and (b). (d) Phase contrast image of the cross-sectioned primo vessel. The genuine primo vessel was stained with Alcian blue, whereas the other part, aggregation of lymphocytes, was not.
