Long-Term Stimulation with Electroacupuncture at DU20 and ST36 Rescues Hippocampal Neuron through Attenuating Cerebral Blood Flow in Spontaneously Hypertensive Rats
The effect of electroacupuncture on the number of opening microvessels in rat hippocampus. (a) Representative immunohistochemistry images at the end of observation in rat hippocampus of Wistar (a1), Wistar + Sham (a2), Wistar + EA (a3), SHR (a4), SHR + Sham (a5), and SHR + EA (a6) group, respectively. Bar = 50 μm. b1–b6, high magnification of a1–a6, respectively. Arrows indicate opening microvessels. Bar = 200 μm. (b) Quantitative evaluation of CD31-positive opening microvessels. Wistar: Wistar rats without any treatment. Wistar + Sham: Wistar rats with stimulation at nonacupoints. Wistar + EA: Wistar rats with stimulation at acupoints. SHR: SHR without any treatment. SHR + Sham: SHR with stimulation at nonacupoints. SHR + EA: SHR with stimulation at acupoints. Data were expressed as mean ± SD from six animals. * versus Wistar group; # versus SHR group; versus SHR + Sham group.