A Methanol Extract of Brugmansia arborea Affects the Reinforcing and Motor Effects of Morphine and Cocaine in Mice

Figure 2

Effects of B. arborea on the acquisition and reinstatement of morphine-induced CPP. Mice were conditioned with 30 or 60 mg/kg of B. arborea (B30 and B60), 20 or 40 mg/kg of morphine (M20 and M40), or 20 or 40 mg/kg of morphine plus 30 or 60 mg/kg of B. arborea (M40 + B30, M40 + B60, M20 + B30, and M20 + B60). Bars represent mean (±standard error of the mean) time spent in the drug-paired compartment before conditioning session (white), after conditioning session (black), during the last extinction session (light gray), and during the reinstatement test (dark gray). After extinction of CPP, mice performed the reinstatement test 15 min after a priming injection of 50%, 25%, or 12.5% of the morphine dose employed for conditioning. ^∗∗∗𝑃<0.001; ^∗∗𝑃<0.01; ^∗𝑃<0.05, significant difference with respect to preconditioning values; ⁺⁺𝑃<0.01; ⁺𝑃<0.05, significant difference with respect to the previous extinction values.