Figure 6: DBT induces EPO expression in cultured HEK293T cells. Cultured HEK293T cells were treated with 0.7 mg/mL of different DBT for 48 hours. The level of EPO mRNA was revealed by real-time PCR, while 18S rRNA was used as an internal control for normalization. The overlayering of mineral oil served as a positive control. Values are expressed as the percentage of increase to basal reading (untreated culture) and are in mean ± SD, where , each with triplicate samples. .