Antiobesity Effects of the Ethanol Extract of Laminaria japonica Areshoung in High-Fat-Diet-Induced Obese Rat

Table 2

Primer sequences.

Target	Primer	Sequence (5′–3′)	Amplicon size; (bp)
PPARα	F	TGACCAGCCTGATGGAGTTA	239
	R	ACTGATAGTGGGATTCTTATTGGG
PPARγ	F	TTCAGTTTGGAGACTTCGGACC	183
	R	TAGGCTCCTGCCAGATTACTCC
ATGL	F	CTCCCAAACTGACCCTTAAACG	194
	R	AGACACTTTCTCCCAGTGGACC
HSL	F	GCTCCCATCGTCAAGAATC	262
	R	TAAAGCGAATGCGGTCC
UCP1	F	CAGAGAGTTTGTCCTCTGGTGC	265
	R	GAAAAATCAAGGGTCCTGCCCC
LPL	F	ATTCTGCACTGCGCAAAGTAC	181
	R	AGTAAAACAAAAGAGCGGCAA
SCD-1	F	CACATCAACTTCACCACGTTCTTC	74
	R	GAAACTTTCTTCCGGTCGTAAGC
CPT1 (muscle)	F	CAATGGTGGGATGAGAGAAC	175
	R	AACCCAGAACTTTAACCTCAA
CPT1 (liver)	F	AACCTTGGCTGCGGTAAGACTA	184
	R	AGTGGGACATTCCTCTCTCAGG
ACO	F	CCAACAAGGTGACATGCTGTGT	184
	R	ATTCGCATTGTGAGAGCAGTTC
UCP3	F	GTTTTGCTGATCTCCTCACCTTT	242
	R	GAACTGCTTGACAGAGTCATAGAGG
SREBP-1c	F	GATGCCAACCAGATTCCCTAAG	210
	R	TCAGTTGTTTCTTTGCCTTCCA
ACC-1	F	TCTATTCGGGGTGACTTTC	109
	R	CTATCAGTCTGTCCAGCCC
ACC-2	F	GGAACTCACGCAGTTGACCAGG	299
	R	CACATAAACCTCCAGGGACGCC
FAS	F	CTGGACTCGCTCATGGGTG	111
	R	CATTTCCTGAAGTTTCCGCAG
DGAT	F	AGACTAGGAGGAGTGTGCAGGC	212
	R	CGCTTCTTCCAAGGGAACTATG
AGPAT	F	GCATTTCAGGATCTCGTTCACA	197
	R	ATCAACCCAACGAGAGCACTTT
GPAT	F	TGATCAGCCAGGAGCAGCTG	508
	R	AGACAGTATGTGGCACTCTC
UCP2	F	GCATTGCAGATCTCATCACTTTCC	222
	R	AGCCCTTGACTCTCCCCTTG
β-actin	F	GCTCTTTTCCAGCCTTCCTT	259
	R	TGATCCACATCTGCTGGAAG

PPARα: peroxisome proliferator-activated receptor α; PPARγ: peroxisome proliferator-activated receptor γ; ATGL: adipose triglyceride lipase; HSL: hormone sensitive lipase; UCP1: uncoupling protein 1; SCD-1: stearoyl-CoA desaturase-1; CPT-1: carnitine palmitoyltransferase-1; ACO: acyl CoA oxidase; UCP3: uncoupling protein 3; SREBP-1c: sterol regulatory element-binding protein-1c; ACC1: acetyl-CoA carboxylase1; ACC2: acetyl-CoA carboxylase2; FAS: fatty acid synthase; DGAT: diacylglycerol O-acyltransferase; AGPAT: acylglycerol-3-phosphate-O-acyltransferase; GPAT: glycerol-3-phosphate-acyltransferase; UCP2: uncoupling protein 2.