Methanol Extract of Artemisia apiacea Hance Attenuates the Expression of Inflammatory Mediators via NF-κB Inactivation
Figure 1
Effect of methanol extracts of Artemisia apiacea Hance (MEAH) on cell viability and NO production. Raw264.7 cells were incubated with 10–300 μg/mL MEAH for 24 h (a) or pretreated with 10–100 μg/mL MEAH for 1 h and then continuously exposed to 1 μg/mL LPS for 24 h (b). After incubation, cell viability was assessed by the MTT analysis. Data represent mean ± S.D. of three separated experiments (significant as compared with vehicle-treated control cells, **; cell viability of control = 100%). N.S.: not significant. (c) NO production. Raw264.7 cells were pretreated with 10–100 μg/mL MEAH for 1 h and then further incubated with 1 μg/mL LPS for 24 h. NO release in the medium was measured by using Griess reagent. Data represent mean ± S.D. of three separated experiments (significant as compared with control cells, **; significant as compared with LPS-treated cells, ##).
