6-Shogaol Protects against Oxidized LDL-Induced Endothelial Injruries by Inhibiting Oxidized LDL-Evoked LOX-1 Signaling
Figure 6
Effects of 6-shogaol, oxidized low-density lipoprotein (oxLDL), and Z-VEID-FMK on caspase-3 activity, cell apoptosis. Human umbilical vein endothelial cells (HUVECs) were pretreated with caspase-3 inhibitor DEVD-CHO (25 μmol/L), for 1 hour, and then exposed to 30 μM 6-Shogaol, 200 μg/mL oxLDL, or a combination of 6-Shogaol and oxLDL. Caspase-3 activity was determined by a fluorogenic assay (a). Apoptotic cells were quantified using flow cytometry (b). Each value represents the mean ± s.e.m. for . The symbols * and #, respectively, indicate that the values significantly differ from control and oxLDL-treated groups, . The symbol Δ means that the value significantly differs from the 6-shogaol + oxLDL-treated groups, Δ.
