503521.fig.007a
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503521.fig.007b
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503521.fig.007c
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Figure 7: Effects of 6-shogaol on oxidized low-density lipoprotein (oxLDL)-induced adherence of THP-1 cells to HUVECs and NF-κB activation and adhesion molecule expression in human umbilical vein endothelial cells. HUVECs were incubated for 2 h in the absence or presence of 6-shogaol or anti-LOX-1 monoclonal antibody (mAb; 40 μg/mL), followed by the addition of 200 μg/mL oxLDL. As positive control, HUVECs were also incubated for 1 h with 10 μM pyrrolidine dithiocarbamate (PDTC) before incubation with oxDL. Nuclear p65 DNA binding activity was measured by ELISA. Each value represents the mean ± s.e.m. for . (a) Effects of 6-shogaol on NF-κB activation in human umbilical vein endothelial cells. (b) Concentration-response effects of 6-shogaol on adhesion molecule expression in human umbilical vein endothelial cells. (c) Effects of 6-shogaol on oxidized low-density lipoprotein (oxLDL)-induced adherence of THP-1 cells to HUVECs. Each value represents the mean ± s.e.m. for . The symbols * and #, respectively, indicate that the values significantly differ from the control and oxLDL-treated groups, .