Decursin and Doxorubicin Are in Synergy for the Induction of Apoptosis via STAT3 and/or mTOR Pathways in Human Multiple Myeloma Cells
Figure 3
Effect of decursin on doxorubicin-induced apoptosis in multiple myeloma cells. U266 or MM.1S cells were treated with decursin (80 M) and/or doxorubicin (1 M) for 24 h. (a) Top panel: morphological changes of U266 cells were observed under inverted microscope (×200). Bottom panel: U266 cell death was determined by the calcein-AM-based live/dead assay as described in Section 2. Red highlights are dead cells, and green highlights live cells. Percent of dead cells was represented. (b) U266 cells were labelled with TdT-mediated dUTP nick end labeling (TUNEL) and analyzed by flow cytometry. (c) U266 or MM.1S cells were stained with PI after fixing in 75% ethanol. DNA contents of sub-G1 were analyzed by flow cytometry. A representative result of three independent experiments is shown for each experiment. Data are presented as means ± SD for triplicate experiments. *, and **, versus untreated control. #, and ##, versus doxorubicin-treated cells.
