Inhibitory Effect of Chrysanthemum zawadskii Herbich var. latilobum Kitamura Extract on RANKL-Induced Osteoclast Differentiation

Figure 3

Effects of CZE on the expression of osteoclast differentiation marker genes. BMMs were cultured with RANKL and M-CSF treatment in the presence or absence of CZE (25 μg/mL) for 4 days. The expression of osteoclast differentiation marker genes was measured by real-time PCR. The expression of mRNA levels was normalized with GAPDH and described as fold change of mRNA level. Data are expressed as the mean ± SD and are representative of at least three independent experiments. *𝑃<0.05, and ^†𝑃<0.01 versus control (0 μg/mL CZE).

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