Inhibitory Effect of Chrysanthemum zawadskii Herbich var. latilobum Kitamura Extract on RANKL-Induced Osteoclast Differentiation
Figure 4
Effects of CZE on RANKL-induced intracellular signaling and the expression of transcription factors in osteoclasts. BMMs were treated with M-CSF and RANKL in the presence or absence of CZE (25 μg/mL) for the indicated time. Lysate (30 μg) was subjected to SDS-PAGE and analyzed by immunoblotting. (a) MAPK (ERK, JNK, and p38) activation was measured by using their respective antibodies. (b)-(c) The expression of c-Fos and NFATc1 was detected by anti-c-Fos and NFATc1 antibody, respectively. Fold change normalized by actin is presented in the right panel. Data are representatively obtained from three independent experiments and are expressed as the mean SD. *, and † versus control (0 μg/mL CZE).
