Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 2

Research Article

Reishi Protein LZ-8 Induces FOXP3+ Treg Expansion via a CD45-Dependent Signaling Pathway and Alleviates Acute Intestinal Inflammation in Mice

Figure 2

CD18-dependent IL-2 production is crucial for LZ-8-induced Treg expansion. Primary human CD4+ T cells were pretreated with or without (a, b) anti-IL-2 antibodies (αIL-2; 1, 5, 10 μg/mL) or (c, d) anti-CD18 antibodies (5, 10 μg/mL) for one hour prior to rLZ-8 stimulation for 72 h. (a, c) The cells were then harvested for flow cytometry analysis of CD25 and FOXP3 expression. (b, d) The levels of IL-2 and IL-10 in the culture supernatants were measured by ELISA. The asterisks indicate significant differences (* ; ** ; *** ) between the different treatments.

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