Anti-Inflammatory and Neuroprotective Effects of Constituents Isolated from Rhodiola rosea
Figure 4
Effects of R. rosea constituents on the LPS-induced expressions of TNF-α, IL-1β, and IL-6 in microglial cells. BV2 microglial cells were treated with the Rhodiola rosea constituents (1, 10, 50 μg/mL) 30 min prior to activation by 100 ng/mL LPS for 18 h. Cell extracts were collected from cultured microglia after activation by LPS with treatment of R. rosea constituents, and immunoblot analysis was performed using TNF-α, IL-1β, and IL-6. R. rosea suppressed the expression of TNF-α, IL-1β, and IL-6 in activated microglia, respectively. GAPDH was used as an internal control. Results are representative of three independent experiments. Data were analyzed by one-way ANOVA for multiple comparison and Student-Newman-Keuls test as post hoc test. # as compared with the vehicle group; * as compared with the LPS treated group.
