Aqueous Extract of Solanum nigrum Leaves Induces Autophagy and Enhances Cytotoxicity of Cisplatin, Doxorubicin, Docetaxel, and 5-Fluorouracil in Human Colorectal Carcinoma Cells
Figure 1
Cytotoxicity of AE-SN in DLD-1 and HT-29 human colorectal carcinoma cells (a); DLD-1 cells were treated with 0.05 to 5 mg/mL AE-SN for 24 or 48 hr; (b), HT-29 cells were treated with 0.05 to 5 mg/mL AE-SN for 24 hr; the cytotoxicity of (a) and (b) was analyzed by MTT assay; (c)DLD-1 or HT-29 cells were treated with 0.2 to 1 mg/mL AE-SN; and the cytotoxicity was analyzed by a trypan blue exclusion test. Experiments were performed in triplicate and data are shown as mean ± SD. Both cell lines showed dose-dependent effects with AE-SN treatment at 24 and 48 hr in the MTT assay and trypan blue exclusion test (one-way ANOVA, ). AE-SN: aqueous extract of Solanum nigrum: MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.
