Aqueous Extract of Solanum nigrum Leaves Induces Autophagy and Enhances Cytotoxicity of Cisplatin, Doxorubicin, Docetaxel, and 5-Fluorouracil in Human Colorectal Carcinoma Cells
Figure 4
Cytotoxicity of a combination of AE-SN and the chemotherapeutic drugs, cisplatin, doxorubicin, docetaxel, or 5-Fu. (a), (b), (c), and (d), DLD-1 cells were treated with 0 to 100 μM cisplatin, 0 to 10μM doxorubicin, 0 to 10 nM docetaxel, 0 to 100 μM 5-Fu in combination with 0, 0.2 or 5 mg/mL AE-SN for 48 hr; (e), (f), (g), and (h) HT-29 cells were treated with 0 to 100 μM cisplatin, 0 to 10μM doxorubicin, and 0 to 10 nM docetaxel, or 0 to 100 μM 5-Fu in combination with 0, 0.5 or 1 mg/mL AE-SN for 48 hr. Cytotoxicity was analyzed by MTT assay. Experiments were performed in triplicate and the data shown are mean ± SD. AE-SN: aqueous extract of Solanum nigrum; MTT: 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide.
