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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 514908, 18 pages
http://dx.doi.org/10.1155/2013/514908
Research Article

NBM-T-L-BMX-OS01, Semisynthesized from Osthole, Is a Novel Inhibitor of Histone Deacetylase and Enhances Learning and Memory in Rats

1Department of Biotechnology and Animal Science, College of Bioresources, National Ilan University, Ilan 260, Taiwan
2New Drug Research & Development Center, NatureWise Biotech & Medicals Corporation, Taipei 112, Taiwan
3Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei 110, Taiwan
4Institute of Traditional Medicine, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan
5Graduate Institute of Acupuncture Science, China Medical University, Taichung 404, Taiwan
6Department of Education and Research, Taipei City Hospital, Taipei 103, Taiwan

Received 10 December 2012; Revised 19 February 2013; Accepted 25 February 2013

Academic Editor: Alfredo Vannacci

Copyright © 2013 Ying-Chen Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

NBM-T-L-BMX-OS01 (BMX) was derived from the semisynthesis of osthole, isolated from Cnidium monnieri (L.) Cuss., and was identified to be a potent inhibitor of HDAC8. This study shows that HDAC8 is highly expressed in the pancreas and the brain. The function of HDAC8 in the brain has not been adequately studied. Because BMX enhances neurite outgrowth and cAMP response element-binding protein (CREB) activation, the effect of BMX on neural plasticity such as learning and memory is examined. To examine declarative and nondeclarative memory, a water maze, a passive one-way avoidance task, and a novel object recognition task were performed. Results from the water maze revealed that BMX and suberoylanilide-hydroxamic-acid-(SAHA-) treated rats showed shorter escape latency in finding the hidden platform. The BMX-treated animals spent more time in the target quadrant in the probe trial performance. An analysis of the passive one-way avoidance results showed that the BMX-treated animals stayed longer in the illuminated chamber by 1 day and 7 days after footshock. The novel object recognition task revealed that the BMX-treated animals showed a marked increase in the time spent exploring novel objects. Furthermore, BMX ameliorates scopolamine-(Sco-) induced learning and memory impairment in animals, indicating a novel role of BMX in learning and memory.