NBM-T-L-BMX-OS01, Semisynthesized from Osthole, Is a Novel Inhibitor of Histone Deacetylase and Enhances Learning and Memory in Rats
Effects of SAHA and BMX on water-maze memory acquisition and retrieval in rats. Control, SAHA-, and BMX-treated rats were subjected to water-maze training. The escape latency(s) of each water-maze learning trial ((a), upper) and the mean escape latency of each learning day ((a), lower) were recorded. On day 5, the hidden platform was removed, and the rats were allowed to swim for the probe trial test for 2 min. The time spent in each quadrant was recorded ((b), upper). The representative swimming pattern from the probe trial test is shown ((b), lower). We analyzed the swimming speed (c) and the escape latency of each water-maze learning trial in the visible platform (d) to rule out any motor, visual, or motivation side effect of BMX. After the experiment, the hippocampus was dissected for western blotting of histone 4 lysine 12 acetylation (AcH4K12). A representative gel pattern and quantitative analysis of AcH4K12 and the H4 level in the hippocampus (e). Quantitative analysis of HDAC8 activity in the hippocampus after the probe trial test (f). The data are the mean ± SEM. *, in the comparison of the control group; #, in the comparison between groups. ; **. A two-way ANOVA followed by Newman-Keuls comparisons was applied for (a) and (b). A one-way ANOVA followed by Newman-Keuls comparisons was applied for (c), (d), (e), and (f).