Curcumin Protects against 1-Methyl-4-phenylpyridinium Ion- and Lipopolysaccharide-Induced Cytotoxicities in the Mouse Mesencephalic Astrocyte via Inhibiting the Cytochrome P450 2E1
Figure 3
Cur decreased ROS and MDA productions in primary mesencephalic astrocytes. (a) DCFH-DA fluorescence (green) images of the primary cultured mesencephalic astrocytes. (b) Quantification of DCFH-DA fluorescence intensity. (c) MDA assay in medium of the mesencephalic astrocytes. The primary cultured mesencephalic astrocytes were nonpretreated or pretreated with vehicle (DMSO) or Cur (3 μM) for 30 min and then incubated with PBS, MPP+, or LPS for 48 h. The cells were loaded with DCFH-DA (50 μM final concentration) in DMEM for 30 min in the dark and fixed by 4% formaldehyde. After rinsing cells with PBS three times, cells were observed using a fluorescent microscope (magnification 200x). MDA amount was measured by using the MDA diagnostic kit. Data are presented as mean ± SD of three independent experiments, and compared to the control group; compared to the MPP+ or LPS treatment alone group.
