Curcumin Protects against 1-Methyl-4-phenylpyridinium Ion- and Lipopolysaccharide-Induced Cytotoxicities in the Mouse Mesencephalic Astrocyte via Inhibiting the Cytochrome P450 2E1

Figure 5

Cur inhibited the CYP2E1 expression and activity in the primary mesencephalic astrocytes. (a) Effect of Cur on CYP2E1 mRNA level. (b)-(c) Effect of Cur on CYP2E1 protein level (immunocytochemistry images and the optical density). (d) Effect of Cur on CYP2E1 activity. Primary cultured mesencephalic astrocytes were nonpretreated or pretreated with DMSO or Cur (3 μM) for 30 min and then incubated with PBS, MPP⁺, LPS, or EtOH for 48 h. EtOH was a positive CYP2E1 inducer. CYP2E1 mRNA level was determined with real-time PCR. CYP2E1 protein level was determined with immunocytochemistry described in Section 2 (magnification 200x). And CYP2E1 activity was measured by hydroxylation rate of p-nitrophenol. Data are presented as mean ± SD of three independent experiments, ^∗𝑃<0.05,^∗∗𝑃<0.01, compared to the control group;  ^#𝑃<0.05,^##𝑃<0.01 compared to the MPP⁺, LPS, or EtOH treatment alone group.

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