Cartilage Protective and Chondrogenic Capacity of WIN-34B, a New Herbal Agent, in the Collagenase-Induced Osteoarthritis Rabbit Model and in Progenitor Cells from Subchondral Bone
Figure 2
Effects of WIN-34B on cartilage protection in the knees of collagenase-induced arthritis rabbits. (a) Representative Safranin O stained sections of knee joints from the normal, vehicle, WIN-34B 100 mg/kg, WIN-34B 200 mg/kg, WIN-34B 400 mg/kg, CEL 200 mg/kg, ETCP 400 mg/kg, and Gluco-Hcl 1500 mg/kg group. (b) Proteoglycan content, expressed as a ratio, was calculated by dividing the Safranin O staining intensity (SOI) in the superficial zone (SOI-S) by SOI in the deep zone (SOI-D). (c) Representative Masson’s trichrome stained sections of knee joints from the normal, vehicle, WIN-34B 100 mg/kg, WIN-34B 200 mg/kg, WIN-34B 400 mg/kg, CEL 200 mg/kg, ETCP 400 mg/kg and Gluco-Hcl 1500 mg/kg group. (d) Collagen content, expressed as a ratio, was calculated by dividing the Masson’s trichrome staining intensity (MTI) in the superficial zone (MTI-S) by MTI in the deep zone (MTI-D). Values are mean ± SEM. # compared to the normal group, * and ** compared to the vehicle group. Two independent experiments were performed with similar results.
