Cartilage Protective and Chondrogenic Capacity of WIN-34B, a New Herbal Agent, in the Collagenase-Induced Osteoarthritis Rabbit Model and in Progenitor Cells from Subchondral Bone
Figure 3
Effects of WIN-34B on chondrogenic differentiation in the knees of collagenase-induced arthritis rabbits. (a) Representative immunostained stained sections of cartilage from knee joints of animal in the normal, vehicle, and WIN-34B 200 mg/kg group. Lane 1: rabbit anti-CD105 antibody stained sections, lane 2: rabbit anti-CD73 antibody stained sections, lane 3: rabbit-anti-type II collagen (Col II) antibody stained sections, lane 4: rabbit-anti-aggrecan antibody stained sections. (b) Intensity of positive stained area of anti-CD105, anti-CD73, anti-type II collagen, and anti-aggrecan by immunostaining in the cartilage zone of knee joints. (c) Representative immunostained stained sections of subchondral bone from the knee joints of animals in the normal, vehicle, and WIN-34B 200 mg/kg groups. Lane 1: rabbit anti-CD105 antibody stained sections, lane 2: rabbit anti-CD73 antibody stained sections. (d) Intensity of positive stained area of anti-CD105 and anti-CD73 in the subchondral bone zone of knee joints. Values are the mean ± SEM. ## compared to the normal group, ** and *** compared to the vehicle group. Two independent experiments were performed with similar results.
