A Method for the Observation of the Primo Vascular System in the Thoracic Duct of a Rat
Figure 4
Morphological features of a primo vessel (inside the two dotted lines) in a thoracic duct. (a) Phase contrast microscopic image of the primo vessel. (b) Rod-shaped nuclei longitudinally arranged along the primo vessel (inside the yellow-lined region) stained with DAPI. (c) f-actin signals in the cell plasma of the primo vessel stained with phalloidin. (d) Confocal laser scanning microscopic (CLSM) image of the f-actin signals (green) and the nuclei (blue) of the primo vessel. The under and the side panels show cross-sectional views. Rod-shaped nuclei (arrows) are longitudinally arranged along the primo vessel, and lymphocytes (double arrows) are aggregated around the primo vessel.
