Okadaic Acid, a Bioactive Fatty Acid from Halichondria okadai, Stimulates Lipolysis in Rat Adipocytes: The Pivotal Role of Perilipin Translocation

Figure 5

Effects of inhibitors of PKA, PKG, and PKC on okadaic acid-induced lipolysis in rat adipocytes. Cells were incubated with inhibitor alone or okadaic acid (1 μM) in the absence or presence of inhibitors of PKA (KT-5720; 0.3 μM), PKG (KT-5823; 3 μM), or PKC (calphostin C; 0.5 μM) for 2 h at 37°C; media were then collected and glycerol release was measured. ^∗∗∗𝑃<0.001 compared to the DMSO-treated group, ^###𝑃<0.001 compared to the KT-5720-treated group, ⁺⁺⁺𝑃<0.001 compared to the KT-5823-treated group, and ^†††𝑃<0.001 compared to the calphostin-C-treated group.