Resveratrol Downregulates Interleukin-6-Stimulated Sonic Hedgehog Signaling in Human Acute Myeloid Leukemia
Figure 3
Regulation of IL-6 and resveratrol in Shh signaling in AML HL-60 cells. (a) RT-PCR assay of Shh and Gli-1 expressed in HL-60 cells with the treatment of IL-6 (5 and 10 ng/mL) for 24 h. (b) Western blot analysis of Shh preproproteins (Pre-Shh, 45 kDa), C-terminal (C-Shh, 25 kDa), and N-terminal (N-Shh, 20 kDa) peptides in IL-6-treated HL-60 cells with or without MRA (100 nM) treatment. (c) Effect of resveratrol on Shh expression in IL-6-treated HL-60 cells. The relative values were normalized to the internal control, were calculated from three independent experiments, and were expressed as mean ± SE. as compared to the control group. as compared to the IL-6 alone group.
