Destruxin B Isolated from Entomopathogenic Fungus Metarhizium anisopliae Induces Apoptosis via a Bcl-2 Family-Dependent Mitochondrial Pathway in Human Nonsmall Cell Lung Cancer Cells
Figure 2
Destruxin B induces a caspase-dependent cell death. (a) Antiproliferative effect of destruxin B. Human nonsmall cell lung cancer A549 cells were administrated with different concentrations of destruxin B (0, 1, 5, 10, 20, and 30 μM) for 48 h. The cellular morphology was observed under microscope and the cell numbers were then counted by trypan-blue dye exclusion method using hemocytometer. (b) Sub-G1 population is increased by destruxin administration. A549 cells were treated with different concentrations of destruxin B (0, 10, 20, and 30 μM) for 48 h. Cells were then fixed with ethanol and subjected to flow cytometry assay. (c) Destruxin B induces caspase activation. A549 cells were treated with 20 μM destruxin B for 48 h. Cell lysates were harvested and the indicated caspase activity was measured as described in Section 2. (d) Inhibition of caspase activity blocks destruxin-B-triggered cell death. A549 cells were pretreated with indicated caspase inhibitors for 1 h and then treated with 20 μM destruxin B for another 48 h. The cell viability was determined by trypan-blue dye exclusion methods ( versus control).
