In Vitro Evaluations of Cytotoxicity of Eight Antidiabetic Medicinal Plants and Their Effect on GLUT4 Translocation
Cytotoxic effects of plant extracts tested on cells from HepG2 and L6myc cell lines. L6-GLUT4myc cells and HepG2 cells were seeded in 96-well plate (20,000 cells/well), exposed to Allium sativum (a), Allium cepa (b), Trigonella foenum-graecum (c), Olea europaea (d), Nigella Sativa (e), Urtica pilulifera (f), Atriplex halimus (g), and Cinnamomum zeylanicum (h) for 24 h. Cytotoxicity was measured by LDH leakage assay and MTT assay. Each point represents the mean of the data from three independent experiments; bars represent the standard error (S.E) relative to the control. In order to decrease the -axis scale in the LDH presented calculated results, a 100% (control value) was subtracted from all the results shown.