Cytotoxic Activity of Kenaf Seed Oils from Supercritical Carbon Dioxide Fluid Extraction towards Human Colorectal Cancer (HT29) Cell Lines
Annexin V-PI flow cytometry analysis of HT29 cells treated with 0 μg/mL, 100 μg/mL, 200 μg/mL, 500 μg/mL, and 1000 μg/mL of KSO-SFE. The cells were classified into four stages: viable, early apoptosis, late apoptosis, and necrosis/secondary necrosis. Values are expressed as mean ± SD. * compared to untreated cells.