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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 569737, 8 pages
http://dx.doi.org/10.1155/2013/569737
Research Article

Medicinal Fungus Antrodia cinnamomea Inhibits Growth and Cancer Stem Cell Characteristics of Hepatocellular Carcinoma

1Cancer Center, Taipei Veterans General Hospital, Taipei 11217, Taiwan
2Institute of Traditional Medicine, School of Medicine, National Yang Ming University, Taipei 11221, Taiwan
3Department of Chemical and Material Engineering, Chang Gung University, Kwei-Shan, Tao-Yuan 333, Taiwan
4Department of Education and Research, Taipei City Hospital, Taipei 10341, Taiwan
5Department of Radiation Oncology, Mackay Memorial Hospital, Taipei 10449, Taiwan

Received 1 January 2013; Accepted 16 January 2013

Academic Editor: Hui-Fen Liao

Copyright © 2013 Yu-Ming Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Antrodia cinnamomea is an edible fungus commonly used in Asia as a well-known medicinal herb capable of treating drug intoxication and liver cancer. Methods. This study evaluated the anticancer activity of its biotechnological product, mycelial fermentation broth (AC-MFB) on hepatocellular carcinoma (HCC) by tetrazolium-based colorimetric assay in vitro and syngeneic Balb/c 1MEA.7R.1 tumor implantation model in vivo. Given that cancer stem cell characteristics, such as angiogenesis, invasiveness, and migration, are known to cause recurrence, we further evaluated the effect of AC-MFB on cellular viability inhibition of HCC cells, angiogenic activity and migration of endothelial cells, and the release of proangiogenic factors from HCC cells. Results. We found that AC-MFB markedly inhibited the growth of HCC without hepatic enzyme abnormality. This anti-HCC activity was validated by growth-inhibitory effects on both cultured murine 1MEA.7R.1 and human HA22T/VGH HCC cells. For cancer stem cell characteristics, AC-MFB inhibited the cellular viability, migration, and tube formation activity of EA. hy926 and SVEC4-10 endothelial cells. Production of extracellular vascular endothelial growth factor and intracellular hypoxia-inducible factor-1 alpha from HCC cells was suppressed by AC-MFB. Conclusion. Antrodia cinnamomea could inhibit the growth and cancer stem cell characteristics of HCC cells.