Huang Qi Jian Zhong Pellet Attenuates TNBS-Induced Colitis in Rats via Mechanisms Involving Improvement of Energy Metabolism

Figure 6

Energy status of colonic mucosa and Western blot analysis of ATP5D, AMPK-α, and p-AMPK-α. (a) The concentration of ATP in colonic mucosa (𝑛=8). (b) The ratios of ADP/ATP and AMP/ATP (𝑛=8). (c) Representative Western blot of ATP5D, AMPK-α, p-AMPK-α and GAPDH (𝑛=5). (d) Quantitative analysis of ATP5D protein (𝑛=5). (e) Quantitative analysis of AMPK-α and p-AMPK-α proteins. (f) The ratio of p-AMPK-α/AMPK-α (𝑛=5). Data were mean ± SEM. *𝑃<0.05 versus Sham group; ^#𝑃<0.05 versus TNBS 15 d group.

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