Figure 4: Western blot analysis for verification of the representative differentially expressed proteins. Forty micrograms of each tested protein extracted from the knee synovial tissues of normal rats, vehicle-treated CIA rats (CIA), and QFGJS-treated CIA rats (CIA + QFGJS) were separated on 12.5% SDS gels and probed with a specific primary antibody of Pgk1, Gstp1, Aldh6a1, or vimentin. Beta-actin was used as loading control and normalization. The Western blot was a representative of three individual experiments ( ).