The Predicted Proteomic Network Associated with the Antiarthritic Action of Qingfu Guanjieshu in Collagen-II-Induced Arthritis in Rats
Western blot analysis for verification of the representative differentially expressed proteins. Forty micrograms of each tested protein extracted from the knee synovial tissues of normal rats, vehicle-treated CIA rats (CIA), and QFGJS-treated CIA rats (CIA + QFGJS) were separated on 12.5% SDS gels and probed with a specific primary antibody of Pgk1, Gstp1, Aldh6a1, or vimentin. Beta-actin was used as loading control and normalization. The Western blot was a representative of three individual experiments ().