Gene Expression Profile of the A549 Human Non-Small Cell Lung Carcinoma Cell Line following Treatment with the Seeds of Descurainia sophia, a Potential Anticancer Drug
Figure 2
Dose-dependent gene expression by EEDS treatment in A549 cells. (a) Approximately 5,400 differentially expressed genes with a fold ratio greater than 2 or less than 0.5 (for up- and down-regulation, resp.) compared to the vehicle control group in at least one sample were clustered hierarchically. Columns and rows represent individual samples and genes, respectively. The expression ratio color scale ranges from red (high) to green (low), as indicated by the scale bar. Genes exhibiting statistically significant dose-dependent alterations were identified via the STEM program (). (b) The Down-pattern is composed of 1,680 genes and the Up-pattern is composed of 1,673 genes. (c) Data acquired from expression chip analysis was confirmed by semi-qPCR. Ten selected genes displaying Up- () and Down- () patterns were amplified using gene specific primers. β-actin and GAPDH were used as loading controls.
