Aconiti Lateralis Preparata Radix Activates the Proliferation of Mouse Bone Marrow Mesenchymal Stem Cells and Induces Osteogenic Lineage Differentiation through the Bone Morphogenetic Protein-2/Smad-Dependent Runx2 Pathway
Figure 2
mBMMSC isolation and characterization using FACS analysis of surface markers and multilineage mesenchymal stem cell differentiation. (a) FACS analysis using positive (CD105, Sca-1) and negative (CD11b) selections. (b) The differentiation of mBMMSCs cells examined at 21 days following treatment shows the stimulation of osteogenesis and adipogenesis. Left panel: cells were stained with Alizarin red and von Kossa to identify osteogenic differentiation. To recognize adipogenic differentiation, lipid vesicles were stained with Oil Red: magnification, 40x. Right panel: mRNA levels of osteoblast- and adipocyte-specific markers such as osteocalcin and lipoprotein lipase were analyzed using RT-PCR.
