Aconiti Lateralis Preparata Radix Activates the Proliferation of Mouse Bone Marrow Mesenchymal Stem Cells and Induces Osteogenic Lineage Differentiation through the Bone Morphogenetic Protein-2/Smad-Dependent Runx2 Pathway
Figure 3
(a) The effect of ALR on the growth of mBMMSCs. “Normal” represents PBS-treated cells, columns represent the mean ± SD (), and * indicates that the mean is significantly different from the control value (**). (b) Proliferating cell nuclear antigen (PCNA) immunoreactivity measurements. Cells are stained with either anti-PCNA antibodies (green) or propidium iodide (red) for nuclear detection. Overlay images of both stains are also presented. bFGF was used as a positive control. All images were obtained using 20x magnification in an Olympus BX-61 fluorescent microscope. (c) The effect of ALR on the progression of mBMMSCs cell cycle. Results are the mean of three independent experiments.
