Resveratrol Impedes the Stemness, Epithelial-Mesenchymal Transition, and Metabolic Reprogramming of Cancer Stem Cells in Nasopharyngeal Carcinoma through p53 Activation
Figure 4
Resveratrol could reverse the metabolic reprogramming of CSCs. (a) Resveratrol (50 μM) increased O2 consumption and (b) repressed the lactate production of TW01 CSCs. (c) Intracellular H2O2 was upregulated in TW01 CSCs exposed to serial doses of resveratrol and 5 Gy irradiation (IR) measured by H2DCFDA staining. (d) Serial doses of resveratrol depolarized the mitochondrial membrane in TW01 CSCs with or without 5 Gy irradiation. The percentage of mitochondrial membrane potential depolarization was measured by the JC-1 green fluorescence shift. In (c) and (d) charts, TW01 CSCs with resveratrol treatment were compared with those of TW01 CSCs without resveratrol treatment. TW01 CSCs with both resveratrol and irradiation treatment were compared with those of TW01 CSCs with irradiation but without resveratrol treatment. (*; **; ***).
