The Preventive Effect of Biochanin A on Bone Loss in Ovariectomized Rats: Involvement in Regulation of Growth and Activity of Osteoblasts and Osteoclasts

Table 3

Bone-related parameters of serum and urine in rats after treatment.

Treatment group; (𝑛=10)	Inorganic phosphate; (mg/dL)	Calcium; (mg/dL)	TNF-α; (pg/mL)	IL-1β; (pg/mL)	DPD/creatine; (nM/mM)
Sham	7.2±0.8	7.4 ± 1.3	20±2.1^b	23±4.9^b	92±6.1^b
OVX	7.3±1.2	7.7 ± 1.5	76±4.3^a	44±5.6^a	148±8.5^a
OVX + E2	7.0±1.1	7.3 ± 1.7	10±1.9^b	18±5.1^b	82±4.1^b
OVX + BCA	7.1±0.7	7.4 ± 1.2	11±1.6^b	20±2.3^b	79±5.2^b

Values are expressed as means ± SD. Differences among treatment groups were evaluated using a one-way ANOVA followed by Fisher’s test. ^a𝑃<0.05, when compared with the Sham groups; ^b𝑃<0.05, when compared with the OVX group. OVX: ovariectomized.