Table of Contents Author Guidelines Submit a Manuscript
Corrigendum

A corrigendum for this article has been published. To view the corrigendum, please click here.

Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 594906, 12 pages
http://dx.doi.org/10.1155/2013/594906
Research Article

Low-Level Laser Stimulation on Adipose-Tissue-Derived Stem Cell Treatments for Focal Cerebral Ischemia in Rats

1Department of Neurosurgery, Taichung Veterans General Hospital, Taichung 40705, Taiwan
2Department of Physical Therapy, Hung Kuang University, Taichung 43302, Taiwan
3Department of Medicine, National Defense Medical Center, Taipei 114, Taiwan
4Tri-Service General Hospital, National Defense Medical Center, Taipei 114, Taiwan
5Graduate Institute of Pharmaceutical Science and Technology, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan
6Department of Medical Imaging and Radiological Sciences, Central Taiwan University of Science and Technology, Taichung 40601, Taiwan

Received 4 October 2013; Revised 9 November 2013; Accepted 10 November 2013

Academic Editor: Karl Wah-Keung Tsim

Copyright © 2013 Chiung-Chyi Shen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study investigated the effects of large-area irradiation from a low-level laser on the proliferation and differentiation of i-ADSCs in neuronal cells. MTT assays indicated no significant difference between the amount of cells with (LS+) and without (LS−) laser treatment ( ). However, immunofluorescent staining and western blot analysis results indicated a significant increase in the neural stem-cell marker, nestin, following exposure to low-level laser irradiation ( ). Furthermore, stem cell implantation was applied to treat rats suffering from stroke. At 28 days posttreatment, the motor functions of the rats treated using i-ADSCs (LS+) did not differ greatly from those in the sham group and HE-stained brain tissue samples exhibited near-complete recovery with nearly no brain tissue damage. However, the motor functions of the rats treated using i-ADSCs (LS−) remained somewhat dysfunctional and tissue displayed necrotic scarring and voids. The western blot analysis also revealed significant expression of oligo-2 in the rats treated using i-ADSCs (LS+) as well as in the sham group ( ). The results demonstrated that low-level laser irradiation exerts a positive effect on the differentiation of i-ADSCs and can be employed to treat rats suffering from ischemic stroke to regain motor functions.