Tanshinone IIA and Cryptotanshinone Prevent Mitochondrial Dysfunction in Hypoxia-Induced H9c2 Cells: Association to Mitochondrial ROS, Intracellular Nitric Oxide, and Calcium Levels

Figure 1

Effects of TIIA and CT on hypoxia-induced H9c2 cell injury. (a) Cell viability by MTT assay. The cell viability of normoxia was adjusted to 100% (𝑛=5), ^##𝑃<0.01  versus hypoxia, ***𝑃<0.001  versus normoxia. (b) LDH release. The LDH release of normoxia was adjusted to 100% (𝑛=3), Δ𝑃<0.05versus CT,  ^##𝑃<0.01versus hypoxia, and ^###𝑃<0.001 versus hypoxia, ***𝑃<0.001versus normoxia. (c) Cellular ATP contents. The cellular ATP contents of normoxia was adjusted to 1 (𝑛=6), *𝑃<0.05  versus normoxia, *𝑃<0.05  versus normoxia, ^#𝑃<0.05versus hypoxia, and  ^Δ𝑃<0.05versus CT.

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