Gallic Acid Induces a Reactive Oxygen Species-Provoked c-Jun NH2-Terminal Kinase-Dependent Apoptosis in Lung Fibroblasts
Figure 2
Involvement of JNK in gallic acid-elicited p53 accumulation and apoptosis-related molecule expression. (a) MLFs were pretreated with the indicated concentrations of SP600125 for 1 h and then incubated with 50 g/mL gallic acid for 1 h. Cell lysates were analyzed by Western blot with antibodies against p53. (b) MLFs were pretreated with SP600125 for 1 h and then incubated with 50 g/mL gallic acid for 24 h. Cell lysates were analyzed by Western blot with antibodies against PUMA and Fas. -Actin was used as an internal loading control.
