Triptolide Prevents Bone Destruction in the Collagen-Induced Arthritis Model of Rheumatoid Arthritis by Targeting RANKL/RANK/OPG Signal Pathway
Triptolide inhibits osteoclastogenesis in the coculture system of human fibroblast-like synovial cells (HFLS) and peripheral blood mononuclear cells (PBMCs). (a) Numerous tartrate-resistant acid phosphatase- (TRAPs) positive multinucleated cells considered as osteoclasts were formed in the coculture system of HFLS and PBMCs, while few TRAP-positive multinucleated cells were formed when concentration of 28 nmol/L triptolide were added into this coculture system; (b) The number of osteoclasts per area counted under a light microscope; (c) Expression levels of RANKL and RANK proteins in PBMCs and HFLS measured by Western blot. Soluble OPG concentration in the culture supernatant of HFLS was measured by ELISA. Data are represented as the mean ± SD (). , and in comparison with the vehicle control.