Inhibitory Effects of Traditional Herbal Formula Pyungwi-San on Inflammatory Response In Vitro and In Vivo

Table 3

Changes in the numbers of mast cells, COX-2-, iNOS-, and TNF-α-immunoreactive cells in hind paw skins.

Cells	Control	CA	Dexamethasone	PWS 0.3 g/kg	PWS 1.0 g/kg
Dorsum pedis skin
Mast cells	49.20 ± 9.42	16.80 ± 6.57^a	38.00 ± 8.17^bc	31.60 ± 4.22^ac	40.00 ± 6.20^c
COX-2 + cells	17.20 ± 3.11	43.00 ± 9.97^a	18.00 ± 1.58^c	30.20 ± 6.76^ac	22.60 ± 3.78^c
iNOS + cells	10.20 ± 2.39	111.60 ± 40.34^d	22.00 ± 3.87^df	50.80 ± 14.31^df	27.20 ± 8.17^df
TNF-𝛼 + cells	6.60 ± 2.07	95.80 ± 14.70^a	31.40 ± 10.16^ac	66.40 ± 11.22^ac	32.40 ± 12.99^ac
Ventrum pedis skin
Mast cells	44.60 ± 7.50	11.40 ± 2.30^a	33.00 ± 7.91^ac	25.80 ± 6.57^ac	31.80 ± 4.21^ac
COX-2 + cells	8.60 ± 1.95	43.80 ± 6.50^a	15.60 ± 2.30^bc	30.00 ± 7.45^ac	15.20 ± 1.92^bc
iNOS + cells	23.20 ± 6.06	135.80 ± 37.18^d	32.20 ± 17.60^f	65.40 ± 18.56^df	36.60 ± 11.15^ef
TNF-𝛼 + cells	5.80 ± 1.64	118.00 ± 28.28^d	28.80 ± 9.60^df	74.00 ± 10.89^df	30.40 ± 2.41^df

Values are expressed as mean ± S.D. of five rat hind paws, cells/mm² of dermis.
CA: carrageenan; PWS: Pyungwi-san; COX-2: cyclooxygenase-2; iNOS: inducible nitric oxide synthase; TNF-𝛼: tumor necrosis factor-𝛼.
^a𝑃<0.01 and ^b𝑃<0.05 as compared with intact control by LSD test.
^c𝑃<0.01 as compared with CA control by LSD test.
^d𝑃<0.01 and ^e𝑃<0.05 as compared with intact control by MW test.
^f𝑃<0.01 as compared with CA control by MW test.