Ethanol Extracts of Fresh Davallia formosana (WL1101) Inhibit Osteoclast Differentiation by Suppressing RANKL-Induced Nuclear Factor-κB Activation
Figure 2
Separation of active compounds from WL1101. Two active compounds were isolated from WL1101. The structures of WL13 and WL14 were shown in (a). (b) HPLC was used to isolate the peak compounds. (c) Osteoclast precursors isolated from long bone of adult male rats were incubated in the presence of mouse recombinant soluble RANKL (5 ng/mL) and murine M-CSF (20 ng/mL) with or without diverse pure compound for 5 days. It was found that WL13 and WL14 can potently inhibit RANKL-induced osteoclastogenesis. Each value represents mean ± S.E.M. (); compared with control vehicle.
