Ethanol Extracts of Fresh Davallia formosana (WL1101) Inhibit Osteoclast Differentiation by Suppressing RANKL-Induced Nuclear Factor-κB Activation
Figure 4
WL1101 exerts no significant effect on calcium deposition and cell viability in primary cultured rat osteoblasts. (a) For calcium deposition analysis, primary osteoblasts were cultured in 24-well plates and exposed to WL1101 in the presence of L-ascorbic acid and β-glycerophosphate for 14 days. Long-term treatment with WL1101 exerted no effects on the mineralized deposition of osteoblasts on day 14. The quantitative data are shown in (b) (). (c) For cell viability assay, primary osteoblasts were cultured in 24-well plates and incubated with WL1101 for 2 days. Treatment with WL1101 at 20–200 μg/mL did not affect cell viability (). Each value represents the mean ± SEM.