A Network Study of Chinese Medicine Xuesaitong Injection to Elucidate a Complex Mode of Action with Multicompound, Multitarget, and Multipathway

Figure 2

The effect of ingredients of XST on the protein expression of iNOS in LPS-stimulated RAW 264.7 cells. (a) The cells were incubated with LPS (200 ng/mL) and ginsenoside Rg1, ginsenoside Rb1, notoginsenoside R1, ginsenoside Rd, and ginsenoside Re at 50 μM for twenty-four hours. (b) Cells were incubated with LPS (200 ng/mL) and indicated concentrations of ginsenoside Rd for twenty-four hours. The whole cell extracts were prepared, and the expression level of iNOS was determined by Western blot analysis. The values are expressed as the mean ± S.D. from three independent experiments. Statistical significance: *𝑃<0.05 and **𝑃<0.01 versus LPS-stimulated cells and ^#𝑃<0.05 and ^##𝑃<0.01 versus control (nonstimulated cells).

(a)

(b)