Increased Nerve Growth Factor Signaling in Sensory Neurons of Early Diabetic Rats Is Corrected by Electroacupuncture
Figure 3
Confocal microscopy of TrkA and double immunofluorescence in the basal epidermal layer. To allow better vesicles visualization, the red signal was changed to green by colour palette modification. First column DAPI/TrKA (blue, red palette assigned), second column (green palette assigned), third column DAPI/TrKA/p75 (MERGE). The amount of cytoplasmatic immunoreactive vesicles increases in the basal epidermal layer of the STZ group (first row, arrows) on very low fluorescence background. After EA treatment and the cytoplasmatic immunoreactive vesicles were decreased (second row, arrows), while, in the basal epidermal layer of the control group only few immunoreactive vesicles were detected (third row, arrows). TrkA did not show variations in the three experimental groups. TrkA immunoreactivity is characterized by a medium-high tissue fluorescence background decorated by many immunoreactive vesicles which often were confluent. Scale bar: 8 μm.
