A Taiwanese Propolis Derivative Induces Apoptosis through Inducing Endoplasmic Reticular Stress and Activating Transcription Factor-3 in Human Hepatoma Cells
Figure 7
Overexpression of ATF-3 resulted in enhancement of cell apoptosis in human hepatoma cells treated with the propolis derivative, GS-002. (a) Hep3B cells were transfected with various doses of the pCI-ATF3 plasmid for 24 h, and total cell lysates were used to detect the ATF-3 protein level by Western blotting. (b) and (c) Hep3B cells were transfected with 2 μg of the pCI-ATF3 plasmid for 24 h and then treated with GS-002 for another 24 h. (b) The viable cell number was determined by a crystal violet dye. Values are presented as the mean ± SE of triplcate tests. * versus individual pcDNA3-transfected control cells. (c) Total cell lysates were used to detect protein expression of the full-length PPAR (FU-PPAR), cleaved form of PPAR (CF-PPAR), cleaved form of caspase-3 (CF-caspase-3), cleaved form of caspase-9 (CF-caspase-9), ATF-3, and α-tubulin by Western blotting.
