Acetonic and Methanolic Extracts of Heterotheca inuloides, and Quercetin, Decrease CCl₄-Oxidative Stress in Several Rat Tissues

Table 4

Effect of extracts of H. inuloides and quercetin, on the antioxidant enzymes in lung tissue of Wistar rats untreated and CCl₄ treated at dose of 1.5 mL/kg for three consecutive days.

Group	CAT k/mg protein	SOD U/mg protein	GPx U/mg protein	GR U/mg protein	GST 𝜇mol conjugado CDNB/min/mg protein
O.O	0.120 ± 0.01	51.15 ± 1.3	0.036 ± 0.001	0.105 ± 0.012	0.241 ± 0.002
P.B	0.100 ± 0.02	48.36 ± 1.5	0.032 ± 0.001	0.101 ± 0.014	0.229 ± 0.003
Ac.E	0.090 ± 0.01	44.31 ± 1.1	0.027 ± 0.001	0.090 ± 0.011	0.211 ± 0.002
Me.E	0.095 ± 0.01	49.14 ± 0.9	0.023 ± 0.002	0.095 ± 0.010	0.214 ± 0.003
Quer	0.101 ± 0.01	45.10 ± 1.1	0.030 ± 0.001	0.100 ± 0.014	0.226 ± 0.001
CCl4	0.056 ± 0.01^●,+	22.70 ± 1.2*	0.019 ± 0.001*	0.053 ± 0.011^#,‡	0.152 ± 0.004*
CCl4 + Ac.E	0.080 ± 0.001	34.40 ± 1.7	0.025 ± 0.002	0.082 ± 0.014	0.198 ± 0.003
CCl4 + Me.E	0.089 ± 0.01	37.17 ± 1.2	0.024 ± 0.001	0.088 ± 0.007	0.209 ± 0.003
CCl4 + Quer	0.092 ± 0.01	40.84 ± 2.0	0.022 ± 0.001	0.092 ± 0.005	0.229 ± 0.001

Olive oil (O.O), 0.1 mL/kg; phosphate buffer (P.B), 0.1 mL/kg; acetonic extract (Ac.E) 100 mg/kg p.o; methanolic extract (Me.E) 100 mg/kg p.o; quercetin (Quer)/P.B, (100 mg/kg/200 𝜇L p.o); Ac.E + CCl₄, corresponded to acetonic extract for 6 days in combination with CCl₄ (100 mg/kg p.o, 1.5 mL/kg i.p); Me.E + CCl₄, corresponded to methanol extract for 6 days in combination with CCl₄ (100 mg/kg p.o, 1.5 mL/kg i.p); Quer + CCl₄, (100 mg/kg/200 𝜇L p.o, 1.5 mL/kg i.p) corresponded to quercetin for 6 days in combination with CCl₄ for the last three days of treatment. ^●𝑃<0.01 versus O.O, P.B. Ac.E, Me.E, Quer, CCl₄ + Me.E, Ac.E, CCl₄ + Quer, ⁺𝑃<0.05 versus CCl₄ + Ac.E, *𝑃<0.01 versus all groups, ^#𝑃<0.01 versus O.O, P.B. Ac.E, Me.E, Quer, CCl₄ + Me.E, CCl₄ + Quer, ^‡𝑃<0.05 versus CCl₄ + Ac.E. Each determination was performed twice, in triplicate and the values represent the mean ± SD. 𝑛=3.