Evaluation of a Propolis Water Extract Using a Reliable RP-HPLC Methodology and In Vitro and In Vivo Efficacy and Safety Characterisation

Table 4

The concentration of propolis water (PWE) or ethanolic (PEE) extracts, from Brazilian green propolis, required for a 50% reduction of DPPH or Fe³⁺, representing free radical scavenging and ferric reducing activity, respectively.

Extract	DPPH^• scavening		Fe^3+-Fe^2+ Reducing
	IC50	𝑟^2	IC50	𝑟^2
PEE	56.71 ± 2.31	0.9964	282 ± 9.4	0.9971
PWE	33.36 ± 2.22	0.9988	270 ± 3.27	0.9925

Values are mean ± SD obtained from analyses in triplicate.
The values were expressed as 𝜇g/mL. Lower IC₅₀ values indicate higher antioxidant activity.