Ixeris dentata NAKAI Reduces Clinical Score and HIF-1 Expression in Experimental Colitis in Mice
Figure 3
The effect of Ixeris dentata NAKAI (ID) on dextran-sodium-sulfate- (DSS-) induced cyclooxygenase- (COX-) 2 and hypoxia-inducible-factor- (HIF-) 1α levels in colonic tissues. Ulcerative colitis was induced in female BALC/c mice by administering 5% DSS in the drinking water for seven days. Over the same period, ID (100 mg/kg) and the reference compound sulfasalazine (SFZ; 100 mg/kg) were given orally once daily. COX-2 and HIF-1α levels were determined by western blot analysis. (a) Representative western blot (of three independent experiments) of COX-2 and HIF-1α expression in colonic tissue. (b) Ratios of COX-2/GAPDH and (c) HIF-1α/GAPDH were determined by densitometry. (d) Sections of colons of DSS-treated mice with or without ID treatment were subjected to immunohistochemical analysis. Values represent mean ± S.E.M. Data were analyzed by Student’s test ( versus control and versus DSS alone).
